CircularRNAs (circRNAs) are an intriguing class ofRNAdue to their covalently closed structure, high stability, and implicated roles in gene regulation. Here, we used an exome captureRNAsequencing protocol to detect and characterize circRNAs across >2,000cancersamples. When compared against Ribo-Zero and RNase R, capture sequencing significantly enhanced the enrichment of circRNAs and preserved accuratecircular-to-linear ratios. Using capture sequencing, we built the most comprehensive catalog of circRNA species to date: MiOncoCirc, the first database to be composed primarily of circRNAs directly detected in tumor tissues. Using MiOncoCirc, we identified candidate circRNAs to serve as biomarkers for prostatecancerand were able to detect circRNAs in urine. We further detected a novel class ofcirculartranscripts, termed read-through circRNAs, that involved exons originating from different genes. MiOncoCirc will serve as a valuable resource for the development of circRNAs as diagnostic or therapeutic targets acrosscancertypes.

2000余例肿瘤样本及细胞系（涉及十几种肿瘤类型）中circRNA 的测序结果！